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Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Insert contains SacI (3), SphI (1), and HincII (1) sites.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.1; HincII--4.2, plus smaller; HindIII--4.4; PstI--4.4; BamHI--4.4.
The protein resulting from in vitro translation of this cDNA (unglycosylated, with signal peptide) is precipitated by MAb 17D6 specific to Blast-1.
Detects human genomic restriction fragments of the following sizes (kb): EcoRI--9.3, 6.7, 3.4 (weak); HindIII--2.9, 2.1, 1.2, 0.8.
This is a full-length or close to full-length cDNA clone including 46 bp 5' untranslated and about 430 bp 3' untranslated sequence. The sequence predicts 5 N-glycosylation sites.
The insert is oriented such that transcription from the T7 promoter gives the sense strand.
References
Lai LW, et al. A gene correcting the defect in the CHO mutant Ade-H, deficient in a branch point enzyme (adenylosuccinate synthetase) of de novo purine biosynthesis, is located on the long arm of chromosome 1. Genomics 9: 322-328, 1991. PubMed: 2004783
Fisher RC, Thorley-Lawson DA. Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48). Mol. Cell. Biol. 11: 1614-1623, 1991. PubMed: 1847502
Staunton DE, Thorley-Lawson DA. Molecular cloning of the lymphocyte activation marker Blast-1. EMBO J. 6: 3695-3701, 1987. PubMed: 2828034
