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Acanthamoeba castellanii (Douglas) Page

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編號(hào):B241911

品牌:Mingzhoubio

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產(chǎn)品名稱	Acanthamoeba castellanii (Douglas) Page
商品貨號(hào)	B241911
Strain Designations	CCAP 1501/2B
Application	Biochemical and molecular characterization
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	well, Cincinnati, OH, 1959
Product Format	freeze-dried
Storage Conditions	Freeze-dried: 2°C to 8°C
Axenic/Xenic	Axenic
Type Strain	no
Comments	Experimental pneumonitis and encephalitis Biochemical and molecular characterization DNA, RNA and Protein Sequences phylogeny
Medium	ATCC^® Medium 712: PYG w/ Additives
Growth Conditions	Temperature: 25°C
Cryopreservation	To achieve the best results set up cultures with several different inocula (e.g. 0.25 ml, 0.5 ml, 1.0 ml). Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density. If the cell concentration exceeds the required level do not centrifuge, but adjust the concentration to between 2 x 10⁶ and 2 x 10⁷cysts/ml with fresh medium. If the concentration is too low, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield the desired concentration. While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows: Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath. Allow the DMSO to solidify. Add the required volume of refrigerated medium. Dissolve the DMSO by inverting the tube several times. *NOTE: If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 10⁶ and 10⁷ cells/ml and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.) The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer. To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial. Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 ml of fresh ATCC medium 712 in a T-25 tissue culture flask or plastic 16 x 125 mm screw-capped test tube. Incubate at 25°C.
Name of Depositor	CCAP
Chain of Custody	ATCC <-- CCAP <-- R.J. Duma <-- . . . <-- S.L. Chang
Year of Origin	1959
References	Martinez AJ, et al. Experimental pneumonitis and encephalitis caused by Acanthamoeba in mice: pathogenesis and ultrastructural features. J. Infect. Dis. 131: 692-699, 1975. PubMed: 48530 Chung DI, et al. Biochemical and molecular characterization of a strain KA/S2 of Acanthamoeba castellanii isolated from Korean soil. Korean J. Parasitol. 34: 79-85, 1996. PubMed: 8820744 Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. Byers TJ, et al. Genes of Acanthamoeba: DNA, RNA and protein sequences (a review). J. Protozool. 37: 17S-25S, 1990. PubMed: 1701831 Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 Kong HH, et al. Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea. J. Clin. Microbiol. 40: 1199-1206, 2002. PubMed: 11923331 Reveiller FL, et al. Species specificity of a monoclonal antibody produced to Naegleria fowleri and partial characterization of its antigenic determinant. Parasitol. Res. 86: 634-641, 2000. PubMed: 10952262

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