Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--2.75, 0.56; EcoRI--3.4; PvuII--2.5, 0.52, 0.4; HindIII--3.0, 0.3, + smaller fragments; PstI--3.3. This clone was generated from pYS12. This insert encodes from approximately aa 273 to 498. |
