Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
1903 (?)
Product Format
frozen
Type Strain
no
Comments
Chromosomes
Kinetoplast DNA in dyskinetoplastic strains
Growth Conditions
Duration: in vivo, laboratory rat
Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Subcultivation
Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Name of Depositor
EJ Tobie
Chain of Custody
ATCC <<--EJ Tobie<<--USDA <<--- Pasteur Institut
References
Van der Ploeg LH, et al. Chromosomes of kinetoplastida. EMBO J. 3: 3109-3115, 1984. PubMed: 6526012
Hajduk SL. Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum. Science 191: 858-859, 1976. PubMed: 1251198
Tobie EJ. Loss of the kinetoplast in a strain of Trypanosoma equiperdum. Trans. Am. Microsc. Soc. 70: 251-254, 1951.
